The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).
Hot Start Taq Antibody
The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling.
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