| Specificity |
The product is specific for Bevacizumab. This antibody blocks Bevacizumab binding with VEGF165 (Z02689, VEGF165, Human). The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01895-40, MonoRab Anti-Bevacizumab Antibody (30E1), mAb, Rabbit. |
| Host Species |
Rabbit |
| Immunogen |
Bevacizumab |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.05 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
46E3 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
ELISA binding of MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
In this ELISA assay, MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) was labeled with Biotin.
Coating antigen: Bevacizumab, 1 µg/ml.
MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) dilution start from 2,000 ng/ml.
EC₅₀= 4.421 ng/ml.
MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) blocking with Vascular Endothelial Growth Factor 165 (VEGF 165).
In this ELISA assay, MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) was labeled with Biotin.
Coating antigen: Bevacizumab, 0.3 µg/ml.
MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) final concentration: 8 ng/ml.
VEGF 165 dilution start from 20 µg/ml.
Standard curve of Bevacizumab Sandwich ELISA. The Bevacizumab Sandwich ELISA assay is developed by using MonoRab™ Anti- Bevacizumab Antibody (30E1), mAb, Rabbit (GenScript, A01895-40) and MonoRab™ Anti-Bevacizumab Antibody (46E3) (Genscript, A01962-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRab™ Anti-Bevacizumab Antibody (46E3) (GenScript, A01962-40) was labeled with Biotin.
The sensitivity of detecting Bevacizumab is up to 20 pg/ml.
| Target Background |
Bevacizumab (Avastin) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with colon cancer, lung cancer, glioblastoma, renal-cell carcinoma, and age-related macular degeneration. Bevacizumab is an angiogenesis inhibitorthat blocks angiogenesis by inhibiting vascular endothelial growth factor A (VEGF-A). |
| Synonyms |
Rabbit monoclonal to Avastin |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
