Standard curve of DM1 conjugated Trastuzumab Sandwich ELISA by anti-Human IgG Fc antibody and A02223 in monkey serum.
The DM1 conjugated Trastuzumab Sandwich ELISA assay is developed by using DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.
The samples tested in this assay were DM1 conjugated Trastuzumab. The control samples were not serum spiked. The monkey serum samples were spiked with cynomolgus monkey serum matrix.

Standard curve of DM1 conjugated Trastuzumab Sandwich ELISA by anti-idiotype antibody and A02223.
The Sandwich ELISA assay is developed by using DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) and Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033) and as the capture and detection antibodies, respectively.

In this ELISA assay, Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033) was labeled with HRP. GenScript can provide customized conjugation service for this product per customer's request.

Capture antibody: DM1 Antibody (15L2), mAb, Mouse, 2 μg/ml
Detection antibody: Anti-Trastuzumab Antibody (15H2), mAb, Mouse, 0.156 μg/ml

Standard curve of DM1 conjugated Trastuzumab Sandwich ELISA by anti-idiotype antibody and A02223.
The Sandwich ELISA assay is developed by using DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) and Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) and as the capture and detection antibodies, respectively.

In this ELISA assay, Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) was labeled with HRP. GenScript can provide customized conjugation service for this product per customer's request.

Capture antibody: DM1 Antibody (15L2), mAb, Mouse, 2 μg/ml
Detection antibody: Anti-Trastuzumab Antibody (11C4), mAb, Mouse, 0.026 μg/ml

Standard curve of DM1 Competitive ELISA.
The competitive ELISA assay is developed by using free DM1 block the binding of DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) to HRP conjugated DM1.

Capture antibody: DM1 Antibody (15L2), mAb, Mouse, 2 μg/ml
Detection reagent: HRP conjugated DM1, 4 ng/ml
Free DM1 dilution start from 250 ng/ml.

ELISA binding of DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) with DXd, MMAE, MMAF and DM1 conjugated antibody respectively.

The coating antibody was DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) used at 2 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including DXd, MMAE, MMAF and DM1 conjugated antibodies used at 1 μg/mL concentration. The secondary antibody was Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) used at 0.25 μg/ml concentration.

DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) is specific to DM1 and has no cross-reactivity with MMAE, MMAF, and DXd.

DM1 Antibody (15L2), mAb, Mouse

The product is specific for DM1 and DM1 structurally similar molecules.

产品编号	A02223
规格	40 μg 定制报价
数量
价格	￥1600


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产品概述

Specificity	The product is specific for DM1 and DM1 structurally similar molecules.
Host Species	Mouse
Immunogen	DM1-KLH
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.5-2 µg/ml
Sandwich ELISA	0.5-2 µg/ml
Competitive ELISA	0.5-2 µg/ml

产品属性

Form	Liquid
Storage Buffer	Supplied in PBS, pH 7.4, containing 0.02% ProClin300.
Concentration	0.5 mg/mL
Storage Instructions	The product remains stable up to 1 year at -20 °C from date of receipt. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG1, κ
Clonality	Monoclonal
Clone ID	15L2
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

图例

Standard curve of DM1 conjugated Trastuzumab Sandwich ELISA by anti-idiotype antibody and A02223.
The Sandwich ELISA assay is developed by using DM1 Antibody (15L2), mAb, Mouse (GenScript, A02223) and Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) and as the capture and detection antibodies, respectively.

In this ELISA assay, Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) was labeled with HRP. GenScript can provide customized conjugation service for this product per customer's request.

Capture antibody: DM1 Antibody (15L2), mAb, Mouse, 2 μg/ml
Detection antibody: Anti-Trastuzumab Antibody (11C4), mAb, Mouse, 0.026 μg/ml

技术背景

Target Background

Payloads are the important components of antibody-drug conjugates (ADCs). The most commonly used ADC payloads are MMAE, DM1, and DXd. ADC pharmacokinetic (PK) studies include free payload analysis, conjugated antibody analysis, and total antibody analysis via ligand-binding assay (LBA) and LC-MS/MS assay. The anti-payload antibody is a useful reagent in PK assay for determining conjugated antibodies. It can be used as a capture antibody to determine the conjugated antibody in LBA assay or through immunocapture to enrich the ADC sample in LC-MS/MS assay. The DM1, also known as mertansine, is a microtubule polymerization inhibitor. Trastuzumab emtansine (T-DM1), composed of trastuzumab conjugated to DM1, is a type of targeted cancer drug for breast cancer treatment. T-DM1 is targeted toward HER2 (ERBB2) positive breast cancer.

Synonyms

Mertansine, Maytansinoid DM1, DM 1, 139504-50-0

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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DM1 Antibody (15L2), mAb, Mouse

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