GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.
| Cat. No. | Product Name
| Volume supplied
| Resin content
|
| L00683-12 | High Affinity Ni-charged Resin FF prepacked column (1mlx2) | 1mlx2 | prepacked column |
| L00683-51 | High Affinity Ni-charged Resin FF prepacked column (5mlx1) | 5mlx1 |
| L00683-55 | High Affinity Ni-charged Resin FF prepacked column (5mlx5) | 5mlx5 |
Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.
Characteristics
| Binding capacity | >40 mg of histidine-tagged protein /ml settled resin |
| Matrix spherical | 6% highly cross-linked agarose |
| Average particle size | 90 μm (45-165 μm) |
| Storage solution | 20% ethanol |
| Storage and Stability | 18 months when stored unopened at 2-8 °C |
Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
Reagents Compatible
| Denaturants | Detergents | Reducing agents | Salts | Others |
| 6 M Gu·HCl | 2% Triton X-100 | 20 mM β-ME | 4 M MgCl2 | 50% glycerol |
| 8 M Urea | 2% Tween 20 | 1 mM DTT | 5 mM CaCl2 | 20% ethanol |
| 1% CHAPS | | 2 M NaCl | 1 mM EDTA |
|
