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目录产品 » 蛋白分离纯化 » 蛋白纯化介质 » MonoRabTM Anti-DYKDDDDK Affinity Resin

MonoRab™ Anti-DYKDDDDK Affinity Resin

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.
L00766
￥3740

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Description

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

Key Features
Product content 50% settled resin in TBS with 50% glycerol and 0.02% sodium azide
Matrix 4% cross-linked agarose
Average bead size 90 μm
Ligand MonoRabᵀᴹ monoclonal antibody against DYKDDDDK tag
Binding capacity Approximately 1.5 mg DYKDDDDK-tagged protein (Size: 50KDa) per ml settled resin
Storage and stability Store at -20 °C for up to 12 months.
Resin reuse The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method Alkaline, peptide competitive or SDS-PAGE loading buffer elution*
Reagents compatibility Compatible with commonly used reagents at certain concentrations

*When eluting with SDS-PAGE loading buffer, the heavy chain of the MonoRabTM antibody will be denatured and released from the resin.

Storage & Stability Store at -20 °C for up to 12 months.


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