Benz-Neburase™ Nuclease ELISA Kit

GenScript Benz-Neburase™ Nuclease ELISA Kit is a sensitive assay to detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases. Benz-Neburase™ Nuclease ELISA Kit is based on sandwich ELISA with a detection limit of 8.40 pg/ml. It is a simple, accurate and reproducible solution for residual endonuclease quantitation to add to the purification process, quality control, and release testing. Benz-NeburaseTM Nuclease is genetically engineered endonuclease from S. marcescens that cleaves all forms of DNA and RNA non-specifically, including single stranded, double stranded, linear and circular. They are mostly used to remove residual nucleic acids during production process of biological molecules in biopharmaceutical manufacturing including but not limited to vaccine production, viral vector production, and manufacturing of gene and cell therapy-related products.

产品编号	L00886
规格	96 Test 定制报价
数量
价格	￥7200


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产品概述

Product Description

GenScript Benz-Neburase™ Nuclease ELISA Kit is a sensitive assay to detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases. Benz-Neburase™ Nuclease ELISA Kit is based on sandwich ELISA with a detection limit of 8.40 pg/ml. It is a simple, accurate and reproducible solution for residual endonuclease quantitation to add to the purification process, quality control, and release testing.

产品简介

Linearity Range

15.63 pg/ml-1000 pg/ml

Sensitivity

8.40 pg/ml

Kit Contents

Component	Quantity	Part. No.
Capture Plate	8 wells × 12 strips, 1 plate	L00886 ‐ 80
Benz-Neburase™ Standard (100 ng/ml)	100 μl × 1 vial	L00886 ‐ 10
100 × Detection Reagent	120 μl × 1 vial	L00886 - 20
100 × Enzyme Conjugate	120 μl × 1 vial	L00886 ‐ 30
Sample Dilution buffer	50 ml × 1 vial	L00886 ‐ 60
Assay Buffer A	12 ml × 1 vial	L00886 ‐21
Assay Buffer B	12 ml × 1 vial	L00886 ‐ 31
20 × Wash Solution	40 ml × 1 vial	L00886 ‐ 70
TMB Solution	12 ml × 1 vial	L00886 ‐ 40
Stop Solution	6 ml × 1 vial	L00886 ‐ 50
Plate Sealer	2 pieces	N/A
User Manual	1 copy	N/A

Storage

The unopened kit is stable for at least 12 months from the date of manufacture if stored at 2 °C to 8 °C, and the opened kit is stable for up to 1 month from the date of opening at 2 °C to 8 °C. Do not freeze the reagents in this kit.

应用指导

Detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases.

原理

Assay Principle

When Benz-Neburase™ Standard or testing samples are added to the Capture Plate, Benz-Neburase™ Nuclease in the Standard or testing samples can be captured by the Anti- Benz-Neburase™ Nuclease antibody which is coated on the Capture Plate. Then the Detection Reagent (containing Anti- Benz-Neburase™ Nuclease antibody conjugated with biotin) is added to interact with the Benz-Neburase™ Nuclease bound on the plate. Enzyme Conjugate (Streptavidin-Horseradish Peroxidase conjugate) is added to interact with the biotin conjugated anti- Benz-Neburase™ Nuclease antibody. After the washing steps, TMB Solution (3, 3’, 5, 5’-Tetramethylbenzidine solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color (Optical density, OD) can be measured at 450 nm and 650 nm by a microplate reader. The amount of Benz-Neburase™ Nuclease in the sample is positively proportional with the OD value and can be calculated using a standard curve generated using the Benz-Neburase™ Standard.

图例

Figiure 2: Summary of Assay Procedure

Figure 1: Schematic diagram of Benz-Neburase^TM Nuclease ELISA Kit

For research use only. Not for use in diagnostic procedures.