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DYKDDDDK Peptide

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.

产品编号	RP10586
规格	1 mg 100 mg 大单询价
数量
价格	￥300


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产品概述

Synonyms	DYKDDDDK tag;Flag-tag
Description	A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca²⁺. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
Sequence	{ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS}
Sequence Shortening	DYKDDDDK
Molecular Formula	C₄₁H₆₀N₁₀O₂₀
Molecular Weight	1012.97