Protein A Resin

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.

产品编号	L00210
规格	5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 50 ml Resin (Total 100 ml) 100 ml Resin (Total 200 ml) 200 ml Resin (Total 400 ml) 定制报价
数量
价格	￥1000


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Description

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

产品概述

Product Information

Characteristics of Protein A Resin

Resin Volume	5ml settled resin (10 ml 50% slurry)
Ligand	Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand	5
M.W. of ligand	Approximately 34 kDa
PI of ligand	5.17
Degree of substitution	Approximately 2 mg protein A/ml settled resin
Static binding capacity	> 20 mg human IgG/ml settled resin
Matrix spherical	Agarose, 4%
Average particle size	90 μm (45-165 μm)
Storage solution	20% ethanol
Storage conditions	2-8 °C; Do not freeze

产品属性

Storage & Stability

Store regenerated Protein A Resin in 20% ethanol at 2°C to 8°C. Do not freeze.

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Protein A Resin

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