Protein L Resin

GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

产品编号	L00239
规格	5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 50 ml Resin (Total 100 ml) 100 ml Resin (Total 200 ml) 定制报价
数量
价格	￥1200


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Description

GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein L, first isolated from the surface of bacterial species Peptostreptoccus magnus, binds immunoglobulin through κ light chain interaction. Protein L binds a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

产品概述

Key Features

Characteristics of Protein L Resin

Matrix spherical	4% agarose
Ligand	highly purified protein L
Binding capacity	≥ 15 mg Human IgG/ml settled resin
Average particle size	90 μm (45-165 μm)
Storage solution	1X PBS containing 20% ethanol

产品属性

Storage & Stability

Store regenerated Protein L Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.

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Protein L Resin

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