Figure 1. Schematic diagram of the chemical structure of Ni IDA Resin, Ni IDA Resin FF, Hi affinity Ni-Charged Resin and Hi affinity Ni-Charged Resin FF

High Affinity Ni-Charged Resin FF

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) [1]. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

产品编号	L00666
规格	5 ml Resin (Total 10 ml) 25 ml Resin (Total 50 ml) 100 ml Resin (Total 200 ml) 定制报价
数量
价格	￥403


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Description

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) ^[1]. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

产品概述

Product Information

Cat. No.	Product Name	Volume supplied	Resin content
L00666-5	High Affinity Ni-Charged Resin FF 5 ml	10 ml	50% slurry
L00666-25	High Affinity Ni-Charged Resin FF 25 ml	50 ml	50% slurry
L00666-100	High Affinity Ni-Charged Resin FF 100 ml	200 ml	50% slurry

Key Features

Characteristics of High Affinity Ni-Charged Resin FF

Matrix spherical	6% highly cross-linked agarose
Average particle size	90 µm (45-165 µm)
Dynamic Binding capacity¹	≥50 mg of histidine-tagged protein /mL settled resin
Storage solution	20% ethanol

¹ Dynamic binding capacity conditions:
Sample: 5 mg/mL (histidine)₆-tagged pure proteins (M_r 43 000) in binding buffer (capacity at 10% Breakthrough)
Column volume: 1 mL
Retention time: 3min
Flow rate: 0.333 mL/min
Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4
Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4
Note: Dynamic binding capacity is protein-dependent.

Reagents Compatible with High Affinity Ni-Charged Resin FF

Reducing agents	Denaturants	Detergents	Salts	Others
1 mM DTT	6 M Gua·HCl^†	2% Triton X-100	4 M MgCl₂	50% glycerol
20 mM β-ME	8 M Urea^†	2% Tween 20	5 mM CaCl₂	20% ethanol
5mM TCEP-HCl	——	50% glycerin	2 M NaCl	1 mM EDTA^‡
10 mM reduced glutathione	——	——	100mM Na₂SO₄	60 mM citrate^†

^†Tested for 1 week at 40°C.
^‡The strong chelator EDTA has been used successfully in some cases at 1 mM. Generally, chelating agents should be used with caution (and only in the sample, not in buffers). Any metal ion stripping may be counteracted by addition of a small excess of MgCl2 before centrifugation/filtration of the sample. Note that stripping effects may vary with applied sample volume.

产品属性

Storage & Stability

Store in 20% ethanol at 2°C to 8°C. Do not freeze.

图例

Figure 1. Schematic diagram of the chemical structure of Ni IDA Resin, Ni IDA Resin FF, Hi affinity Ni-Charged Resin and Hi affinity Ni-Charged Resin FF

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High Affinity Ni-Charged Resin FF

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