Streptavidin

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.

产品编号	Z02043
规格	5 mg 定制报价
数量
价格	￥1250


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产品简介

Purity	> 95% as analyzed by SDS-PAGE
Biological Activity	>15 U/mg protein, 1 U is defined as the quantity of protein that binds 1 microgramme
Expression System	E. coli
Theoretical Molecular Weight	13,500 Da
Application	Streptavidin can be used for the coating of solid phases (microtiter plates, beads, etc.) to immobilize or purify biotin-labeled or 2-iminobiotin-labeled molecules. Its conjugation with enzyme or fluorescein is widely used in immunological detection assays, such as the streptavidin/biotin system.
Formulation	Lyophilized from a 0.22 μm filtered solution in 10 mM potassium phosphate buffer, pH 6.5-7.0.
Concentration	Please refer to the COA for the specific lot.
Storage & Stability	The powder may be stored at 4°C for several weeks. For long-term storage, store at -20°C.

技术背景

Target Background

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.

Synonyms

Streptavidin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.