| Species |
Mouse |
| Protein Construction |
Expressed with an N-terminal Met.
IFN-γ (His23-Cys155)
Accession # P01580 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 1 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.15 ng/ml, measured by cytotoxicity assay using WEHI-279 cells. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~15 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
ED₅₀<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
| Target Background |
Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. |
| Synonyms |
Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
