Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.5-5.0 ng/ml.

Lane 1: 3 μg of TPO, His, Human, non-reducing (NR)
Lane 2: 3 μg of TPO, His, Human, reducing (R)
> 95% as analyzed by SDS-PAGE

TPO, His, Human

Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

产品编号	Z03434
规格	10 μg 50 μg 500 μg 1 mg 定制报价
数量
价格	￥1560


联系尊龙凯时人生就是搏

产品简介

Species

Human

Protein Construction

Poly-His	TPO (Ser22-Gly353) Accession # P40225	Poly-His
N-term		C-term

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 1 EU/μg of protein by LAL method

Biological Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The ED₅₀ for this effect is typically 0.5-5.0 ng/ml.

Expression System

Human Cells

Apparent Molecular Weight

75~90 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

图例

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.5-5.0 ng/ml.

Lane 1: 3 μg of TPO, His, Human, non-reducing (NR)
Lane 2: 3 μg of TPO, His, Human, reducing (R)
> 95% as analyzed by SDS-PAGE

技术背景

Target Background

Synonyms

Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growthand development factor; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.