Species |
Human |
Protein Construction |
Expressed with an N-terminal Met.
GM-CSF (Ala18-Glu144) Accession # P04141 |
|
Purity |
> 95% as analyzed by SDS-PAGE |
Endotoxin Level |
< 1 EU/μg of protein by gel clotting method |
Biological Activity |
ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 2.0 × 106 units/mg |
Expression System |
E. coli |
Apparent Molecular Weight |
~14 kDa, on SDS-PAGE under reducing conditions. |
Formulation |
Lyophilized after extensive dialysis against 10 mM PB, pH 7.0. |
Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
GenScript product showed equal activity compared to competitor P
Biological Activity
GM-CSF, Human (Cat. No. Z02695) stimulates cell proliferation of R&D TF-1 cells. The ED50 for this effect is less than 0.5ng/mL.
2 μg of GM-CSF, Human (Cat. No. Z02695) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.
Target Background |
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils. Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. |
Synonyms |
Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; MGC131935; MGC138897 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.