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Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus-producing hepatoma cells.

Mol Med Rep.. 2014-12;  10(6):3113-7
Y Liu, P Gao. Department of Hepatology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
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摘要

The role of annexin II in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) remains to be elucidated. Intracellular hepatitis B surface antigen (HBsAg)-retention contributes to the induction of hepatocarcinogenesis. The present study aimed to investigate the regulation of HBsAg secretion by annexin II expressed in HBV-producing hepatoma cells. The expression of annexin II was analyzed using western blot analysis in SMMC-7721, HepG2, HepG2.2.15, 293T and Chinese hamster ovary (CHO) cells. CHO cells transfected with an annexin II plasmid were used as a positive control. The localization of annexin II and HBsAg was observed in the HepG2 and HepG2.2.15 cells using indirect immunofluorescence. HepG2.... More

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