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Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

medrxiv. 2020-11; 
Julia A Zakashansky, Amanda H Imamura, Darwin F Salgado, Heather C Romero Mercieca, Raphael F L Aguas, Angelou M Lao, Joseph Pariser, Netzahualcóyotl Arroyo-Currás, Michelle Khine
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Molecular Biology Reagents … Aptamers were diluted in phosphate buffer solution. The S1 protein was purchased from GenScript (Cat. No. Z03501) … 6 Coronavirus (COVID-19) Testing - Statistics and Research, //ourworldindata.org/coronavirus-testing, (accessed June 11, 2020) … Get A Quote

摘要

Using the children's toy, Shrinky-Dink ©, we present an aptamer-based electrochemical (E-AB) assay that recognizes the spike protein of SARS-CoV-2 in saliva for viral infection detection. The low-cost electrodes are implementable at population scale and demonstrate detection down to 0.1 fg mL of the S1 subunit of the spike protein.

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