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Organization, genomic targeting, and assembly of three distinct SWI/SNF chromatin remodeling complexes in Arabidopsis

Plant Cell .. 2023-04; 
Wei Fu, Yaoguang Yu, Jie Shu , Zewang Yu, Yixiong Zhong, Tao Zhu, Zhihao Zhang, Zhenwei Liang, Yuhai Cui, Chen Chen , Chenlong Li
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Proteins, Expression, Isolation and Analysis Proteins were loaded onto 4% to 20% gradient protein gels (GenScript, SurePAGE, Cat. No. M00655) and 4% to 20% Precast Protein Plus Gel (Yeasen, Cat. No. 36256ES10) at 120 V for 2 h.For silver staining, samples were run on a 4% to 20% gradient protein gels (GenScript, SurePAGE, Cat. No. M00655) and stained with Fast Silver Stain Kit Beyotime, Cat. No. P0017S) according to the manufacturer’s instructions. Get A Quote

摘要

Switch defective/sucrose non-fermentable (SWI/SNF) complexes are evolutionarily conserved multi-subunit machines that play vital roles in chromatin architecture regulation for modulating gene expression via sliding or ejection of nucleosomes in eukaryotes. In plants, perturbations of SWI/SNF subunits often result in severe developmental disorders. However, the subunit composition, pathways of assembly, and genomic targeting of the plant SWI/SNF complexes are poorly understood. Here, we report the organization, genomic targeting and assembly of three distinct SWI/SNF complexes in Arabidopsis thaliana: BRAHMA-Associated SWI/SNF complexes (BAS), SPLAYED-Associated SWI/SNF complexes (SAS), and MINUSCULE-Associated ... More

关键词

Arabidopsis; BAS; BRAHMA; Chromatin remodeling; MAS; SAS; SWI/SNF complexes.