位点扫描库（Positional Scanning Library）

位点扫描库是多肽序列优化的一个重要工具。在目的位点上替换全部的天然氨基酸，每条多肽替换一次，可用来鉴别活性较佳的多肽序列。常用在复杂的蛋白混合物中鉴定T细胞抗原表位，或用相互依赖的子位点来定位底物，以减少合成和筛选工作量。

应用领域

Overlapping Peptide Alanine Scanning Truncation Library Positional Scanning Random Library Scrambled Library T Cell Truncated Library

参考文献

Pinilla C, Appel JR, Campbell GD, Buencamino J, Benkirane N, Muller S, and Greenspan NS. All-D peptides recognized by an anti-carbohydrate antibody identified from a positional scanning library. J. Mol. Biol. Nov 1998 13; 283(5): 1013-25.



Lee CH, Lee MS, Kim SJ, Je YT, Ryu SH, and Lee T. Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells. Peptides. Oct 2008 17.



Chaparro RJ, Burton AR, Serreze DV, Vignali DA, and DiLorenzo TP. Rapid identification of MHC class I-restricted antigens relevant to autoimmune diabetes using retrogenic T cells. J. Immunol. Methods. Jun 2008 1; 335(1-2): 106-15.



Nino-Vasquez JJ, Allicotti G, Borras E, Wilson DB, Valmori D, Simon R, Martin R, and Pinilla C. A powerful combination: the use of positional scanning libraries and biometrical analysis to identify cross-reactive T cell epitopes. Mol. Immunol. Feb 2004; 40(14-15): 1063-74.



Houghten RA. The broad utility of soluble peptide libraries for drug discovery. Gene. Dec 1993 27; 137(1): 7-11.